Bioenvironmental and Biothreat Detection

For environmental use only. Not for human use.

Diagnostic Kits

SMART ™ II Anthrax Spore and PA (Protective Antigen)

Tests for Bacillus anthracis Spore and PA Lateral Flow Screening Assays sold separately.

For Environmental Use Only. Not for Human Use.

INTENDED USE:

This test is designed to detect Bacillus anthracis spore from environmental samples. It is not intended to be used in the diagnosis of anthrax or any other disease.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Anthrax spore Lateral Flow Test Devices
Plastic Droppers
Chase Buffer

COLLECTION KITS NOT INCLUDED:

(To collect sample, you should use one of the following kits or their equivalent.)

Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004

If the sample has debris or large particles, it may require use of the SPK:

Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100

ASSAY PROCEDURE:

CAUTION: For assay to function properly, NHD’s SWIPE or a validated collection system must be utilized to properly collect and process the sample. Failure to utilize a proper collection system may cause false negative or false positive results.

Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
Open pouch of Anthrax lateral flow device. Remove contents.
Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane
Proven Technology —”Desert Storm Tested”

DOWNLOADS:

Order Anthrax Diagnostic Kits

SMART ™ II Cholera 01 and 0139

Tests for Vibrio cholerae-01 and -0139 are sold separately.

For Environmental Use Only. Not for Human Use.

DESCRIPTION:

Cholera epidemics, caused by V. cholerae serotype O1, continue to be a devastating disease of immense global significance in many developing countries. Clinically, cholera may range from asymptomatic colonization to severe diarrhea with massive fluid loss, leading to dehydration, electrolyte disturbances, and death. V. cholerae O1 causes this secretory diarrhea by colonization of the small intestine and production of a potent cholera toxin. Because of the clinical and epidemiological importance of cholera, it is critical to determine as quickly as possible whether or not the organism from a patient with watery diarrhea is positive for V. cholerae O1.

A fast, simple and reliable method for detecting V. cholerae O1 is a great value for clinicians in managing the disease and for public health officials in instituting control measures. NHD’s monoclonal antibody provides specificity for the cholerae O1 antigen. The Cholera SMART II utilizes a monoclonal antibody based lateral flow format. The monoclonal antibodies are specific for the A antigen of O1 lipopolysaccharide (LPS) of V. cholerae O1. The Cholera SMART II test is simple, and can be performed in approximately 15 minutes.

INTENDED USE:

Cholera SMART ™ II (Sensitive Membrane Antigen Rapid Test) is a rapid colorimetric immunoassay designed for the direct presumptive detection of Vibrio cholerae 01 in human feces as an adjunct to culture methodology for the diagnosis of cholera in symptomatic patients when cholera is endemic or epidemic.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Cholera Lateral Flow Test Devices
Plastic Droppers
Chase Buffer

COLLECTION KITS NOT INCLUDED:

(To collect sample, you should use one of the following kits or their equivalent.)

Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004

If the sample has debris or large particles, it may require use of the SPK:

Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100

ASSAY PROCEDURE:

Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
Open pouch of Cholera lateral flow device. Remove contents.
Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane

DOWNLOADS:

Order Cholera Diagnostic Kits

SMART™ II Ricin

DESCRIPTION:

This test is designed to detect RICIN toxin from environmental samples. It is not intended to be used in the treatment of any human/animal disease or illness.

For Environmental Use Only. Not for Human Use.

INTENDED USE:

Ricin SMART ™ II (Sensitive Membrane Antigen Rapid Test) is a rapid colorimetric immunoassay designed for the direct presumptive detection of Vibrio cholerae 01 in human feces as an adjunct to culture methodology for the diagnosis of cholera in symptomatic patients when cholera is endemic or epidemic.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Ricin Lateral Flow Test Devices
Plastic Droppers
Chase Buffer

COLLECTION KITS NOT INCLUDED:

(To collect sample, you should use one of the following kits or their equivalent.)

Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004

If the sample has debris or large particles, it may require use of the SPK:

Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100

ASSAY PROCEDURE:

Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
Open pouch of Cholera lateral flow device. Remove contents.
Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane

DOWNLOADS:

Ricin Assay Instructions (PDF)

Order Ricin Diagnostic Kits

SMART™ II Cholera Test for Ballast Water

DESCRIPTION:

Cholera has been located globally in all bodies of water. It is important for all water going vessels to determine whether there is any presence of Cholera in the ballast water before exposing it to the environment. The Ballast Water Test utilizes overnight growth of a sample of ballast water to test for the presence of Cholera organisms at the detection rate of one viable Cholera organism in 100ml of ballast water. The test utilizes a lateral flow format with very specific monoclonal antibodies. The SMART TM II Cholera Test for Ballast Water is highly reliable, simple to use, and can be performed on site aboard ship.

For Environmental Use Only. Not for Human Use.

INTENDED USE:

Cholera SMART TM II (Sensitive Membrane Antigen Rapid Test) Ballast Water Test is a rapid, qualitative colorimetric immunoassay in the lateral flow format, designed, when combined with auxiliary reagents for the direct presumptive and qualitative detection of Vibrio cholerae O1 and O139 in ballast water samples.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Each kit contains the following in quantities sufficient to adequately test 10 ballast water samples as specified 5 Cholera O1 tests and 5 Cholera O139 tests. Additional devices or accessory reagents, such as a Positive Control Reagent, can be obtained separately. Each bottle of growth media is used to
test both O1 and O139.

5 FOIL POUCHES: Each contains one SMART TM II Cholera O1 Device.
5 FOIL POUCHES: Each contains one SMART TM II Cholera O139 Devices
1 CHASE BUFFER
5 APW BOTTLES: Each contains 200 ml of APW (2.5X)10 PLASTIC DROPPERS

ASSAY PROCEDURE:

In order to be able to detect viable cholera at the low level of one viable
organism per 100ml of ballast water, it is necessary to transfer the ballast
water in our specially prepared APW, so as to achieve the most probable
number equivalency (MPN)

Preheat the APW media at 37 0 C.
Add 300ml ballast water sample to the preheated APW bottle and gently mix.
Incubate the inoculated sample for 18- 24 hours at 37 0 C.
Place 3 drops or 100 µl (1/10 th ml) of the inoculated sample into the sample well (S) of each of the Cholera LFA O1 and O139 devices.
Wait 2-3 minutes for the inoculated sample to be absorbed into the sample well of the device.
Add 2 free falling drops of the Chase Buffer to the sample well.
Read results after 15 minutes, but no longer than 30 minutes.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane

DOWNLOADS:

SMART II CHOLERA O1/O139 Ballast Water Test Instructions (PDF)

Order Cholera Diagnostic Kits

SMART™ Staph Enterotoxin B

This is a lateral flow screening assay. Not intended for use in the diagnosis or treatment of any human or animal disease or illness.

For Environmental Use Only. Not for Human Use.

FEATURES:

Monoclonal Antibody based assay
99% Specific for Neisseria gonorrhoeae
100% Sensitive
Effective with mixed or non-viable cultures
No heating
Single or Batch testing
No agglutination guesswork
Clear-cut color reaction
Detects GC where routine sugars and FA’s may not, even on cultures in transit over 10 days
COLLECTION KITS NOT INCLUDED

Downloads:

Staph Enterotoxin B (PDF)

Order SEB Diagnostic Kits

SMART II™ Tularemia

Tularemia is a rare infectious disease caused by the bacterium, Francisella tularensis.

For Environmental Use Only. Not for Human Use.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane
COLLECTION KITS NOT INCLUDED

Downloads:

Tularemia (PDF)

Order Tularemia Diagnostic Kits

SMART™ II Yersina Pestis

We sell test devices to detect F1 as well as F1 and V.

For Environmental Use Only. Not for Human Use.

Plague

INTENDED USE:

This test is designed to detect Yersina Pestis from environmental samples. It is not intended to be used in the diagnosis of Yersina Pestis or any other disease.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Yersina Pestis Lateral Flow Test Devices
Plastic Droppers
Chase Buffer

COLLECTION KITS NOT INCLUDED:

(To collect sample, you should use one of the following kits or their equivalent.)

Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004

If the sample has debris or large particles, it may require use of the SPK:

Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100

ASSAY PROCEDURE:

Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
Open pouch of Yersina Pestis lateral flow device. Remove contents.
Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane

DOWNLOADS:

Y Pestis (PDF)

Order Yersina Pestis Diagnostic Kits

SMART™-II Botulism Toxin

This test is designed to detect Botulinum toxin “A” from environmental samples.

For Environmental Use Only. Not for Human Use.

INTENDED USE:

This test is designed to detect Botulism toxin from environmental samples. It is not intended to be used in the diagnosis of botulism or any other disease.

CAUTION:

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.

MATERIALS INCLUDED PER POUCH:

Botulism Toxin Lateral Flow Test Devices
Plastic Droppers
Chase Buffer

COLLECTION KITS NOT INCLUDED:

(To collect sample, you should use one of the following kits or their equivalent.)

Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004

If the sample has debris or large particles, it may require use of the SPK:

Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100

ASSAY PROCEDURE:

Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
Open pouch of Botulism Toxin lateral flow device. Remove contents.
Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.

FEATURES:

Less than 20 seconds technician time
Room temperature storage
Distinct color reaction on capture membrane
Proven Technology — “Desert Storm Tested”

DOWNLOADS:

Botulism toxin (PDF)

Order Botulism Diagnostic Kits

PROFILE® 1

Rapid Bacteria Detection in Under 5 minutes

For Environmental Use Only. Not for Human Use.

DESCRIPTION

The PROFILE® 1 Bioluminometer is a hand-held instrument capable of determining the presence of low levels of bacteria. PROFILE® 1 is able to differentiate microbial from somatic cells, yeast from bacteria, and can eliminate interfering (quenching) substances from the sample. To maximize specificity, a series of simple, patented, sample preparation steps are used to remove ATP arising from human cells and other interfering compounds. PROFILE® 1 will detect only viable organisms. Studies performed by the USDA, Agriculture Canada, DOD, University of Michigan, and others have shown an excellent correlation to standard culture methods. Results are read on the LCD display.

FEATURES

Provides reliable, near real-time detection of bacteria.Accuracy of a 48-hour culture in less than five minutes.Discriminates bacterial ATP from Total ATPEliminates quenching substances from sampleHand-held, battery powered

DOWNLOADS

PROFILE 1 Manual (PDF)

Order PROFILE 1 Bioluminometers

Bioavailable Ferric Iron Assay

The Bioavailable Ferric Iron Assay measures the amount of ferric iron in soil or sediment that can be reduced to ferrous iron (Fe2+) by iron-reducing bacteria. Bioavailable ferric iron is one indicator of natural attenuation and can be important for other in situ bioremediation technologies such as enhanced anaerobic bioremediation.

For Environmental Use Only. Not for Human Use.

DESCRIPTION

Natural attenuation can be a technically, environmentally and economically preferred choice for remediation of hydrocarbon and chlorinated hydrocarbon contaminants at suitable sites. Ferric iron reducing bacteria can degrade benzene, vinyl chloride, and other contaminants if adequate BIOAVAILABLE Iron is present. The test is used in conjunction with other accepted tests to evaluate site suitability for natural attenuation; and for monitoring effectiveness and efficiency of ongoing natural attenuation.

The Bioavailable Ferric Iron Assay measures the amount of ferric iron in soil or sediment that can be reduced to ferrous iron (Fe²⁺) by iron-reducing bacteria. Bioavailable ferric iron is one indicator of natural attenuation and can be important for other in situ bioremediation technologies such as enhanced anaerobic bioremediation.

Developed by Camp Dresser & McKee and available through New Horizons Diagnostics, this Field Test Kit offers direct determination of Bioavailable Iron and is:

PROVEN
ACCURATE
EASY TO USE
COST EFFECTIVE

FEATURES

Does not require special skills or training – simply follow kit protocolUse with equal ease in the field or in the laboratoryKit contains required reagents and equipmentFe II colorimeter optional

DOWNLOADS

Bioavailable Ferric Iron Assay (PDF)

Order Bioavailable Ferric Iron Assays

Bioavailable Ferric Iron Assay
SIMPLE PROCEDURE

STEP 1 (T₀):

STEP 2 (T₃₀):

Bioenvironmental and Biothreat Detection

Diagnostic Kits