SMART™-II Anthrax (Spore)


Bacillus anthracis (spore) Lateral Flow Screening Assay
For Environmental Use Only. Not for Human Use.

Anthrax (PDF)

  • Less than 20 seconds technician time
  • Room temperature storage
  • Distinct color reaction on capture membrane
  • Proven Technology ---"Desert Storm Tested"


This test is designed to detect Bacillus anthracis spore from environmental samples. It is not intended to be used in the diagnosis of anthrax or any other disease.

This test is a rapid, screening assay. No known rapid screening assay is 100% sensitive or 100% specific, therefore results should be confirmed by an alternative method.


  • Anthrax spore Lateral Flow Test Devices
  • Plastic Droppers
  • Chase Buffer

(To collect sample, you should use one of the following kits or their equivalent.)

  • Large Surface Sample Collection Kit, (SWIPE-1), Reorder No. 87-130001
  • Powder/Small Surface Sample Collection Kit, (SWIPE-2), Reorder No. 87-130002
  • Liquid Sample Collection Kit, (SWIPE-3), Reorder No. 87-130003
  • Air Sampler Sample Collection Kit, (SWIPE-4), Reorder No. 87-130004
If the sample has debris or large particles, it may require use of the SPK:
  • Sample Processing Kit: SWIPE-SPK, Reorder No. 87-130100
CAUTION: For assay to function properly, NHD’s SWIPE or a validated collection system must be utilized to properly collect and process the sample. Failure to utilize a proper collection system may cause false negative or false positive results.
  1. Collect samples and follow dilution guidelines to ensure sample is in a liquid form.
  2. Open pouch of Anthrax lateral flow device. Remove contents.
  3. Place three drops (or 100µl) of liquid sample into sample well of a lateral flow device using plastic dropper provided.
  4. Wait three (3) minutes for the sample to be absorbed into the sample well. Then place two (2) free falling drops of Chase buffer from the dropper bottle.
  5. Read results at 15 minutes (no longer than 30 minutes). Observe the development of color on the Control (C) and Test Line (T) and record result. See table to interpret test.